Corrigendum: A polyalanine peptide derived from polar fish with anti-infectious activities

نویسندگان

  • Marlon H. Cardoso
  • Suzana M. Ribeiro
  • Diego O. Nolasco
  • César de la Fuente-Núñez
  • Mário R. Felício
  • Sónia Gonçalves
  • Carolina O. Matos
  • Luciano M. Liao
  • Nuno C. Santos
  • Robert E. W. Hancock
  • Octávio L. Franco
  • Ludovico Migliolo
چکیده

Due to the growing concern about antibiotic-resistant microbial infections, increasing support has been given to new drug discovery programs. A promising alternative to counter bacterial infections includes the antimicrobial peptides (AMPs), which have emerged as model molecules for rational design strategies. Here we focused on the study of Pa-MAP 1.9, a rationally designed AMP derived from the polar fish Pleuronectes americanus. Pa-MAP 1.9 was active against Gram-negative planktonic bacteria and biofilms, without being cytotoxic to mammalian cells. By using AFM, leakage assays, CD spectroscopy and in silico tools, we found that Pa-MAP 1.9 may be acting both on intracellular targets and on the bacterial surface, also being more efficient at interacting with anionic LUVs mimicking Gram-negative bacterial surface, where this peptide adopts α-helical conformations, than cholesterol-enriched LUVs mimicking mammalian cells. Thus, as bacteria present varied physiological features that favor antibiotic-resistance, Pa-MAP 1.9 could be a promising candidate in the development of tools against infections caused by pathogenic bacteria.

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A polyalanine peptide derived from polar fish with anti-infectious activities

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2016